Gram, Gram And C. Gram - 895 Words

Gram’s Stain was discovered in 1882, but published in 1884 by a Danish Bacteriologist Doctor by the name of Hans Christian Gram. Gram stumbled upon this method while he was examining some lung tissue from patients that had died of pneumonia. While examining this lung tissue Gram discovered that certain stains were more favorable and retained by the bacterial cells. It was only a few years later that Gram produced a staining procedure that he divided into two groups. The two groups divided almost all bacteria into what he called Gram positive (purple) and Gram negative (pink). The Gram’s staining procedure is still used today and is the method that forms the basis for identifying bacteria. In order to perform this procedure, you will need a Bunsen Burner or a Tirrill burner, alcohol- cleaned microscope slide, and water. You will also need The following reagents: Crystal violet, Gram’s iodine solution, 95% alcohol, and safranin. These stain reagents are important because they are used to determine the Gram reaction for microorganisms’ identification. Crystal violet will stain the bacterial cell, and Iodine will bind the stain. The alcohol differentiates bacteria retaining or not the crystal violet within the cell wall, and the safranin will be used as a counterstain to stain the bacteria. You are now ready to start you process. First you will need to light your burner to the hottest part of the flame before starting your gram stain. After the burner is lit you will move toShow MoreRelatedIdentify And Describe Three Groups Of Low G + C Gram Positive Bacteria1579 Words   |  7 PagesIdentify and describe three groups of low G+C Gram-positive bacteria. Clostridia: rod-shaped, obligate anaerobes, some form endospores, produce potent toxins that cause a variety of diseases in humans. Important in medicine industry (Botox oral plaque). Examples of clostridia include C. tetani (cause tetanus), C. perfringens ( causes gangrene), C. botulinum ( causes botulism) and C. difficle (severe diarrhea). Microbes related to Clostridium include: Epulopiscium - a giant bacterium that canRead MoreA Study On Nutrient Agar1078 Words   |  5 Pages37 ° C for 48 hours. This allowed for observation of the colony morphology. Separation of Gram-positive and Gram-negative The original unknown culture was streaked for isolation on Columbia CNA agar and MacConkey agar using a quadrant streak, inverted, and placed in a 37 ° C incubator for 48 hours. CNA agar contains a mix of colistin and nalidixic acid, as well as sheep blood. If there is poor or no growth on CNA agar, then the organism was inhibited by colistin and nalidixic acid and is Gram-negativeRead MoreDiffusion And Osmosis Lab Report1012 Words   |  5 Pageshypertonic, and isotonic hypothesis for the solution   made during the study,   four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis. Introduction The studyRead MoreHealthy Nutrition Personal Food Intake Evaluation1003 Words   |  4 Pageswas 29 grams, 3 grams, and 21 grams; my respective breakfast intake of Carbohydrates was 80 grams, 11 grams, and 12 grams; and my respective breakfast intake of total Lipids was 53 grams, 36 grams, and 21 grams (Rinzler, 2004). On the three consecutive recorded days, my respective lunch intake of Protein was 17 grams, 28 grams, and 31 grams; my respective lunch intake of Carbohydrates was 27 grams, 38 grams, and 49 grams; and my respective lunch intake of total Lipids was 33 grams, 56 grams, and 22Read MoreOrganisms Of Two Unknown Bacterial Cultures Essay1700 Words   |  7 Pagesidentify the organisms of two unknown bacterial cultures. Students must identify the species of the unknown bacteria by utilizing the techniques and information learned in previous laboratory exercises. These techniques include streaking for isolation, Gram staining, and specific biochemical tests. Students are giv en a map known as a dichotomous key, a guide in determining the identity of their unknown sample. Identifying microbes using a series of biochemical tests, like those performed by studentsRead MoreLab Report : The Laboratory Experiment1277 Words   |  6 Pagesexpected to be able to correctly isolate and identify the two microorganisms, one Gram-positive and one Gram-negative, from a mixed culture the student receives. The mixed culture I received was number 21. From this unknown mixed culture, the possible Gram-positive organisms include: Clostridium perfringens, Corynebacterium xerosis, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermis. The possible Gram-negative organisms include: Enterobacter aerogenes, Pseudomonas aeruginosa, NeisseriaRead MoreSample Assignment1280 Words   |  6 Pagesoperator from decimal import * def get_top_most_frequent_ngrams(n_grams, f): Get the top f most frequent n-grams sorted_n_grams = sorted(n_grams.items(), key=operator.itemgetter(1)) sorted_n_grams.reverse() return sorted_n_grams[0:f - 1] def extract_character_n_grams(doctext, n): Parse a document text and get all the character n-grams along with their frequencies as a dictionary n_gram_dict = {} i = 0 while (i + n) len(doctext):Read MoreIdentification Of Bacteria Is Important For A Verity Of Reasons1459 Words   |  6 PagesStaphylococcus aureus is a gram-positive cocci although it is not usually harmful infections can occur especially in healthcare settings. This certain strains of this bacteria are resistant to some antibiotics this strain is called MRSA. Staphylococcus epidermidis also a gram-positive cocci and normally does not cause any maladies (Otto). Streptococcus pyogenes is a gram-positive cocci bacteria. Medical implications include necrotising facetious and sore throat. Bacillus subtilis is a gram positive rod bacteriaRead MoreTechniques Used For All Procedures During The Unknowns Exercise906 Words   |  4 Pagesthe quadrant streak technique on two Trypticase Soy Agar (TSA) plates. One of the plates was incubated aerobically and the other was incubated anaerobically, both at 37 ° C for twenty-four hours. Aseptic technique was used for all procedures du ring the unknowns exercise. All incubations during this experiment were set at 37  °C. On the second day of the unknowns experiment, the TSA plates were observed and their results were recorded. The aerobic TSA plate had a medium amount of growth and the coloniesRead MoreNormal Distribution and Multiple Vitamin1319 Words   |  6 Pagesskewed-right with mean = 10 minutes and standard error = 0.8 minutes. b) Distribution is skewed-right with mean = 10 minutes and standard error = 8 minutes. c) Distribution is approximately normal with mean = 10 minutes and standard error = 0.8 minutes. d) Distribution is approximately normal with mean = 10 minutes and standard error = 8 minutes. ANSWER: c 2. Suppose the ages of students in Statistics 101 follow a skewed-right distribution with a mean of 23 years and a standard deviation of 3 years. If we