Gram, Gram And C. Gram - 895 Words

Gram’s Stain was discovered in 1882, but published in 1884 by a Danish Bacteriologist Doctor by the name of Hans Christian Gram. Gram stumbled upon this method while he was examining some lung tissue from patients that had died of pneumonia. While examining this lung tissue Gram discovered that certain stains were more favorable and retained by the bacterial cells. It was only a few years later that Gram produced a staining procedure that he divided into two groups. The two groups divided almost all bacteria into what he called Gram positive (purple) and Gram negative (pink). The Gram’s staining procedure is still used today and is the method that forms the basis for identifying bacteria. In order to perform this procedure, you will need a Bunsen Burner or a Tirrill burner, alcohol- cleaned microscope slide, and water. You will also need The following reagents: Crystal violet, Gram’s iodine solution, 95% alcohol, and safranin. These stain reagents are important because they are used to determine the Gram reaction for microorganisms’ identification. Crystal violet will stain the bacterial cell, and Iodine will bind the stain. The alcohol differentiates bacteria retaining or not the crystal violet within the cell wall, and the safranin will be used as a counterstain to stain the bacteria. You are now ready to start you process. First you will need to light your burner to the hottest part of the flame before starting your gram stain. After the burner is lit you will move toShow MoreRelatedIdentify And Describe Three Groups Of Low G + C Gram Positive Bacteria1579 Words   |  7 PagesIdentify and describe three groups of low G+C Gram-positive bacteria. Clostridia: rod-shaped, obligate anaerobes, some form endospores, produce potent toxins that cause a variety of diseases in humans. Important in medicine industry (Botox oral plaque). Examples of clostridia include C. tetani (cause tetanus), C. perfringens ( causes gangrene), C. botulinum ( causes botulism) and C. difficle (severe diarrhea). Microbes related to Clostridium include: Epulopiscium - a giant bacterium that canRead MoreA Study On Nutrient Agar1078 Words   |  5 Pages37 ° C for 48 hours. This allowed for observation of the colony morphology. Separation of Gram-positive and Gram-negative The original unknown culture was streaked for isolation on Columbia CNA agar and MacConkey agar using a quadrant streak, inverted, and placed in a 37 ° C incubator for 48 hours. CNA agar contains a mix of colistin and nalidixic acid, as well as sheep blood. If there is poor or no growth on CNA agar, then the organism was inhibited by colistin and nalidixic acid and is Gram-negativeRead MoreDiffusion And Osmosis Lab Report1012 Words   |  5 Pageshypertonic, and isotonic hypothesis for the solution   made during the study,   four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis. Introduction The studyRead MoreHealthy Nutrition Personal Food Intake Evaluation1003 Words   |  4 Pageswas 29 grams, 3 grams, and 21 grams; my respective breakfast intake of Carbohydrates was 80 grams, 11 grams, and 12 grams; and my respective breakfast intake of total Lipids was 53 grams, 36 grams, and 21 grams (Rinzler, 2004). On the three consecutive recorded days, my respective lunch intake of Protein was 17 grams, 28 grams, and 31 grams; my respective lunch intake of Carbohydrates was 27 grams, 38 grams, and 49 grams; and my respective lunch intake of total Lipids was 33 grams, 56 grams, and 22Read MoreOrganisms Of Two Unknown Bacterial Cultures Essay1700 Words   |  7 Pagesidentify the organisms of two unknown bacterial cultures. Students must identify the species of the unknown bacteria by utilizing the techniques and information learned in previous laboratory exercises. These techniques include streaking for isolation, Gram staining, and specific biochemical tests. Students are giv en a map known as a dichotomous key, a guide in determining the identity of their unknown sample. Identifying microbes using a series of biochemical tests, like those performed by studentsRead MoreLab Report : The Laboratory Experiment1277 Words   |  6 Pagesexpected to be able to correctly isolate and identify the two microorganisms, one Gram-positive and one Gram-negative, from a mixed culture the student receives. The mixed culture I received was number 21. From this unknown mixed culture, the possible Gram-positive organisms include: Clostridium perfringens, Corynebacterium xerosis, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermis. The possible Gram-negative organisms include: Enterobacter aerogenes, Pseudomonas aeruginosa, NeisseriaRead MoreSample Assignment1280 Words   |  6 Pagesoperator from decimal import * def get_top_most_frequent_ngrams(n_grams, f): Get the top f most frequent n-grams sorted_n_grams = sorted(n_grams.items(), key=operator.itemgetter(1)) sorted_n_grams.reverse() return sorted_n_grams[0:f - 1] def extract_character_n_grams(doctext, n): Parse a document text and get all the character n-grams along with their frequencies as a dictionary n_gram_dict = {} i = 0 while (i + n) len(doctext):Read MoreIdentification Of Bacteria Is Important For A Verity Of Reasons1459 Words   |  6 PagesStaphylococcus aureus is a gram-positive cocci although it is not usually harmful infections can occur especially in healthcare settings. This certain strains of this bacteria are resistant to some antibiotics this strain is called MRSA. Staphylococcus epidermidis also a gram-positive cocci and normally does not cause any maladies (Otto). Streptococcus pyogenes is a gram-positive cocci bacteria. Medical implications include necrotising facetious and sore throat. Bacillus subtilis is a gram positive rod bacteriaRead MoreTechniques Used For All Procedures During The Unknowns Exercise906 Words   |  4 Pagesthe quadrant streak technique on two Trypticase Soy Agar (TSA) plates. One of the plates was incubated aerobically and the other was incubated anaerobically, both at 37 ° C for twenty-four hours. Aseptic technique was used for all procedures du ring the unknowns exercise. All incubations during this experiment were set at 37  °C. On the second day of the unknowns experiment, the TSA plates were observed and their results were recorded. The aerobic TSA plate had a medium amount of growth and the coloniesRead MoreNormal Distribution and Multiple Vitamin1319 Words   |  6 Pagesskewed-right with mean = 10 minutes and standard error = 0.8 minutes. b) Distribution is skewed-right with mean = 10 minutes and standard error = 8 minutes. c) Distribution is approximately normal with mean = 10 minutes and standard error = 0.8 minutes. d) Distribution is approximately normal with mean = 10 minutes and standard error = 8 minutes. ANSWER: c 2. Suppose the ages of students in Statistics 101 follow a skewed-right distribution with a mean of 23 years and a standard deviation of 3 years. If we

The Salem Witch Trials Essay - 1333 Words

The Salem Witch Trials, taking place between February 1692 and May 1693, arose from a period of mass hysteria regarding witchcraft. The puritanical society of New England emphasized a need for a Bible-based society, which caused a fear of the supernatural and gave rise to the false accusations of â€Å"witches.† With testimonies of witches rooted in the Old Testament, the idea of witchcraft eventually made its way into the superstitious and everyday Puritan life, and was fueled by the rejection of the group during the reign of Queen Elizabeth I. The intense paranoia among the Puritans eventually led the group to go as far as even setting their own people on fire may be accounted for by the rejection of the religion itself. They were considered†¦show more content†¦Puritan teachings were influenced by Calvinist theology, leading to the â€Å"dismissal of idolatry and a focus on images drawn from scripture and everyday life.† The Puritans stressed the importance of community, as it was centered on the church and members were encouraged to report the sins of others in order to keep the community pure as a whole. As John Winthrop explained, â€Å"the first governor of the Massachusetts Bay Colony, explained in his letter written on May 22, 1643:† â€Å"Our civil Government is mixt: the freemen choose the magistrates each year and assist [them] in making of laws, imposing taxes, disposing of lands†¦ Our churches are governed by Pastors, Teachers, ruling Elders Deacons, yet the power lies in the whol e congregation.† The Puritans also had strict attitudes and superstitions that lead to religious extremism and false accusations. They believed in â€Å"absolute sovereignty of God† and placed considerable emphasis on scripture. It was â€Å"against the law not to attend church† and any other religion was attributed to Satan, whom the Puritans thought had profound influence on the weak individuals in society (women and children). The importance of having a righteous community influenced the Puritans to turn each other in for sinning, causing paranoia among community members. Witchcraft can be described asShow MoreRelated salem witch trial Essay1180 Words   |  5 Pagessalem witch trial This is about witchcraft and is started like this: In the winter of 1691-92, several people in Salem Village, most of them young women, but eventually including a few men and boys, began behaving in a strange unusual manner†, with an affect which was interpreted as illness. The towns minister, Samuel Parris, whose daughter and niece were among those with this odd affect, sought to cure the perceived problem with prayer; others, including a doctor of physic who was calledRead MoreSalem Witch Trial Hysteria Essay818 Words   |  4 PagesTwenty people were put to death for witchcraft in Salem during the 1692 Salem Witch Trial Hysteria. In The Crucible, a woman, Elizabeth Proctor, gets accused of witchcraft by a young girl by the name Abigail Williams, who just so happens to be having an affair with Elizabeth’s husband, John. Once John finds out Abigail accused his wife, he starts trying to find proof that all of these young girls are pretending that they are being hurt by these older women, just so that they will be hanged. The officialsRead MoreEssay about Salem witch trials1931 Words   |  8 Pages Salem Witch Trials: Casting a spell on the people Today, the idea of seeing a witch is almost inconsequential. Our Halloween holiday marks a celebration in which many will adorn themselves with pointy black hats and long stringy hair, and most will embrace them as comical and festive. Even the contemporary witchcraft religious groups forming are being accepted with less criticism. More recently, the Blair Witch movie craze has brought more fascination than fear to these dark and magical figuresRead MoreEssay on The Theories of the Salem Witch Trials1135 Words   |  5 PagesThe Theories of the Salem Witch Trials The Salem Witch trials and what caused them is very debatable. Some theories lead to Rye poisoning from bread to even people faking it. The most believable claim is that people were faking it. Everyone had a motive and they all just wanted to save themselves. It was a time when people were selfish and only cared for themselves. This time in Salem was a troubling time, making it seem likely that satan was active (Linder). The townsfolk are believed to haveRead MoreThe Salem Witch Trials Essay1369 Words   |  6 PagesThe Salem Witch Trials The witch trials of the late 1600s were full of controversy and uncertainty. The Puritan town of Salem was home to most of these trials, and became the center of much attention in 1692. More than a hundred innocent people were found guilty of practicing witchcraft during these times, and our American government forced over a dozen to pay with their lives. The main reasons why the witch trials occurred were conflicts dealing with politics, religion, family, economicsRead MoreThe Salem Witch Trials Essay2008 Words   |  9 Pageswomen and men. The madness continued for over four months. The notorious witch trials of Salem, Massachusetts occurred from June through September. It is a brief, but turbulent period in history and the causes of the trials have long been a source of discussion among historians. Many try to explain or rationalize the bizarre happenings of the witch hunts and the causes that contributed to them. To understand the trials and how they came to be, we must first examine the ideals and views of the peopleRead MoreSalem Witch Trials Essay1478 Words   |  6 Pages1692 marked a major event in history in the town of Salem, Massachusetts. The Salem Witchcraft Trials still leaves this country with so many questions as to what happened in that small town. With all the documentation and accounts of the story, people are still wondering why 19 people died as a result of these trials. This paper will discuss the events leading up to the Salem Witch Trials and the events that took place during and after the trials, and the men and women who were killed or spent theRead MoreThe Salem Witch Trials Essay1349 Words   |  6 PagesBefore 1692, the supernatural was a part of people’s everyday normal life. This is so as people strongly believed that Satan was present and active on earth. Men and women in Salem Village believed that all the misfortunes that befell them were the work of the devil. For example, when things like infant death, crop failures or friction among the congregation occurred, people were quick to blame the supernatural. This concept first emerged in Europe around the fifteenth century and then spread toRead More Salem Witch Trials Essay876 Words   |  4 PagesSalem Witch Trials The Salem Witch Trials was probably considered the darkest time for the New England Colony. This was a mass murder of women and a few men that were supposed witches. All of this started from two little girls and a bacteria in the bread that affected the brain. This all started on January 20,1692 when nine-year-old Elizabeth Parris and eleven-year-old Abigail Williams started to exhibit strange behavior. Blasphemous screaming, Seizures, Trance-like states and MysteriousRead MoreThe Salem Witch Trials Essay1674 Words   |  7 PagesAlthough many people have their assumptions as to what specifically caused the Salem Witch Trials, no one has a definite account. One must use logic and prior knowledge to come to the conclusion and realize that multiple factors play a key role in causing the trials. The Salem Witch Trials were caused by religion, politics, teenage boredom, family feuds, economic conditions, and fears of the people. The overall effect of the trials was a major part in American history, not only was it a learning experience

My Personal Code of Conduct Being a Nurse

Question: Write an essay on Personal Code of Ethics Statement? Answer: Ethics are actually not optional within healthcare line rather they are essential part to be carried within by every healthcare professional. Being a nurse they are included within my professional life as well. My ethical statements are as follows: Respect of Persons * Uphold a Patient primary Ethics.* I always care for the patients plus the staff along with same level of admiration that I want them to show towards me.* I treat patients along with all kindness, tenderness as well as dignity.* I respect the privacy as well as diffidence of the patients. Respect for the confidentiality * I never share the medical information of patients with anyone except people that are included in the health care and are integral to care of patient.* I never discuss patients otherwise their illnesses within public places.* I never openly identify the patients, in the verbal words otherwise in script, devoid of sufficient explanation.* I never request otherwise permit unlawful people to the patient heed locations of the hospital (Cribb, 2005). Honesty * I always tend to be truthful in the verbal as well as in documented communications.* I do never cheat, plagiarize, and otherwise act dishonestly in my workplace. Responsibility for the Patient Care * I tend to obtain patient's knowledgeable permission for the analytic tests otherwise treatments plus also esteem patient's right towards refusing care and events.* I suppose that patients are my responsibility under care until I hand off care to some other nurse and she acknowledges transfer of the care.* I never develop romantic otherwise sexual relations with the patients; and if any such relation seems to get developed, I seek proper guidance plus terminate professional relationship. Professional Growth plus Awareness of the Limitations * I keep myself aware about my limitations as well as shortages in the acquaintance plus capabilities and also understand when plus whom to seek command from.* I never involve patients within personal issues and also never solicit for the personal gain. Evade Conflicts of the Interest * I try my best to resolve all the clinical conflicts in favor of patient.* While in premises I never accept any gifts of any value from patients.* I never refer patients towards laboratories otherwise other healthcare amenities where they have direct monetary bet without any disclosure (Fulford, Dickenson Murray, 2002). Respect for Personal Ethics * I know that it is not good to perform any procedure like abortions, and extinction of the medical treatment which I, consider are unprincipled, and unlawful, otherwise might be a harmful one towards patients. Respect for the Property plus laws * I always adhere to regulations as well as policies of ACHE Code of Ethics like rules leading the fire security, dangerous waste removal as well as worldwide precautions and safety measures* I adhere to the regional and local, state as well as federal laws plus regulatory standards and maintain them in my professional life Professional Ideals 1. Clinical * I generally cultivate as well as practice the clinical virtues, like caring, empathy as well as compassion.2. Conscientiousness* I try my best to fulfill my professional responsibilities properly and conscientiously.* I always notify answerable supervisor and if anything hinders the capability to carry out the actions efficiently and successfully.* I dedicate myself to a lifelong learning as well as self-development through realizing a proper individual program of anyhow ongoing learning as well as nonstop quality enhancement.* I tend to complete each of my tasks precisely, methodically, and legibly and also in very timely way (GILLON, 2005).3. Collegiality otherwise Cooperation* I always cooperate with my colleagues and coworkers and also with all technical and non-technical staffs of the hospital.* I tend to learn from others and also from my own mistakes.* I tend to be very much generous along with my time while helping and answering questions of patients and co-staf fs, as well as visitors.* I use a communication tools like telephone and emails and letters responsibly as well as equitably.4. Objectivity* I try to avoid providing a professional care towards members of my family otherwise to persons along with whom I have close, and personal relation.5. Responsibility towards Community* I always try to avoid unnecessary monetary expenditures for the patients as I know it would prove me unethical.* I provide proper emergency services towards all patients despite of their capability towards pay (Loewy Loewy, 2004).I tend to follow my ethics and morals and abide by all ethical as well as legal rules in my personal plus professional life. References Cribb, A. (2005). Health and the good society. Oxford: Clarendon Press.Fulford, K., Dickenson, D., Murray, T. (2002). Healthcare ethics and human values. Malden, Mass.: Blackwell Publishers.GILLON, R. (2005). Toleration and Healthcare Ethics. Cambridge Q. Healthcare Ethics, 14(01). doi:10.1017/s0963180105050115Loewy, E., Loewy, R. (2004). Textbook of healthcare ethics. Dordrecht: Kluwer Academic Publishers.

Native by One Republic free essay sample

â€Å"This is life in color.† Ryan Tedder and members of his band, One Republic, at one time liked to consider themselves genreless, balancing both alternative and pop influences in their music. If this was the case in previous albums, Native takes the band in a completely different direction. In this album, One Republic goes for the home run and brings in a full blast of pop brilliance. Most bands adjusting to suit the needs of the mainstream pop radio find themselves in an utter disaster. One Republic however, which already had a foot in the water, finds itself in the ideal situation to succeed. Chart topping singles launch the album with a bang. â€Å"Counting Stars, composed by a series of well developed beats and choruses, positions itself as the album’s superior track. It is followed by tracks â€Å"If I Lose Myself† and â€Å"Feel Again†, both escalated in prominence by Tedder’s rich vocals. We will write a custom essay sample on Native by One Republic or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page For the most part, the first half of the album releases a feel good, early morning vibe. As Native continues, the songs begin to break off and develop their own individual personalities. â€Å"Light it Up† and â€Å"Can’t Stop† act as One Republic’s experimental tracks. Their echoic, swirling melodies add a new flavor to the album. They give way to an especially powerful moment in the album in the form of the track â€Å"Burning Bridges†. Tedder’s vocals give the song depth, causing the listener to not help but feel deeply emotional throughout. The album is continued by a few lighter songs, until it finally reaches the most perhaps its most unappreciated song: â€Å"Life In Color†. Hidden as a bonus track, it emulates earlier hits in nearly every category. Its lowly position on the disk is the band’s only blunder. One Republic’s Native proves to be their finest work to date. Compared to earlier albums, Native is easily the best-rounded. It excels in every way imaginable, including production, vocals, instrumentals, and lyrics. For critics that thought One Republic was geared more toward singles than overall substance, this album certainly puts their beliefs to rest. 5/5 Native by One Republic free essay sample One Republic is one of my favorite band. Few years ago I went to their concert in Prague and I loved it! When they were playing Apologize the whole club was singing with them. So I was really excited about the new album. And I finally got it! And it is exactly how I imagined, maybe even better. One Republic showed their best. The album is full of energy. Some of the songs are slowly some of them are faster. But together it sounds really nice. They are usually singing about love and life and this album is not different. The one that made me almost cried when I heart it for the first time is Counting Stars. Not slow, not fast. Something in the middle and thats just perfect. Feel again this was the first song from the album that was played at the radios. And you could just feet again that this album would be good. We will write a custom essay sample on Native by One Republic or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Preacher slowly, emotional. Singing about how it was when he was a kid and about his dad. Very lovely, it makes me cry too When you compare this album with One Republics older work you would see that there are not much difference. It is still lovely, full of emotion. I like this album. One thing that I would say, that One Republic could have more faster songs.